elisa (bertin pharma Search Results


elisa  (Bertin Pharma)
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elisa kit  (Bertin Pharma)
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Elisa Kit, supplied by Bertin Pharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5-fold concentrated cgrp elisa buffer  (Bertin Pharma)
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(A) Proportions of DRG neurons with <t>CGRP-like</t> immunoreactivity in lumbar sections from Scn11a +/+ and Scn11a +/L799P mice (n = 5–7 mice per group). (B) Sections of lumbar DRGs. The left section contains CGRP-positive neurons (labeled by stars), the right section was processed without anti-CGRP antibody for control. (C) Display of all DRG neurons analyzed for CGRP-like immunoreactivity. Each neuron is defined by its radius and its grey value. Neurons in the left panel, not exposed to the anti CGRP antibody, show the background grey level. Neurons in the other panels were counted as CGRP-positive if they had grey values above background. (D) Protocol of CGRP release experiments from sciatic nerve axons. Values from one nerve of a Scn11a +/+ mouse with 10 −7 M capsaicin are shown as an example. Areas in grey above the x-axis indicate the incubation time (in minutes) in the different test tubes. (E) CGRP release from sciatic nerve axons from naïve Scn11a +/+ mice without and after stimulation with different concentrations of capsaicin (n = 4–6 per concentration). (F) Scn11a +/L799P mice (3–7 months old) showed significantly less CGRP release from sciatic nerve axons in baseline (n = 14–21 nerves per group) and after stimulation with capsaicin (n = 8–11 nerves per group). Values are mean ± SEM. ***p < 0.001. BL baseline, CAPS capsaicin, CGRP calcitonin gene-related peptide, Δ difference.
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elisa kits for ang ii  (Bertin Pharma)
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Bertin Pharma elisa kits for ang ii
(A) Proportions of DRG neurons with <t>CGRP-like</t> immunoreactivity in lumbar sections from Scn11a +/+ and Scn11a +/L799P mice (n = 5–7 mice per group). (B) Sections of lumbar DRGs. The left section contains CGRP-positive neurons (labeled by stars), the right section was processed without anti-CGRP antibody for control. (C) Display of all DRG neurons analyzed for CGRP-like immunoreactivity. Each neuron is defined by its radius and its grey value. Neurons in the left panel, not exposed to the anti CGRP antibody, show the background grey level. Neurons in the other panels were counted as CGRP-positive if they had grey values above background. (D) Protocol of CGRP release experiments from sciatic nerve axons. Values from one nerve of a Scn11a +/+ mouse with 10 −7 M capsaicin are shown as an example. Areas in grey above the x-axis indicate the incubation time (in minutes) in the different test tubes. (E) CGRP release from sciatic nerve axons from naïve Scn11a +/+ mice without and after stimulation with different concentrations of capsaicin (n = 4–6 per concentration). (F) Scn11a +/L799P mice (3–7 months old) showed significantly less CGRP release from sciatic nerve axons in baseline (n = 14–21 nerves per group) and after stimulation with capsaicin (n = 8–11 nerves per group). Values are mean ± SEM. ***p < 0.001. BL baseline, CAPS capsaicin, CGRP calcitonin gene-related peptide, Δ difference.
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elisa (bertin pharma; #a05033)  (Bertin Pharma)
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Bertin Pharma elisa (bertin pharma; #a05033)
(A) Proportions of DRG neurons with <t>CGRP-like</t> immunoreactivity in lumbar sections from Scn11a +/+ and Scn11a +/L799P mice (n = 5–7 mice per group). (B) Sections of lumbar DRGs. The left section contains CGRP-positive neurons (labeled by stars), the right section was processed without anti-CGRP antibody for control. (C) Display of all DRG neurons analyzed for CGRP-like immunoreactivity. Each neuron is defined by its radius and its grey value. Neurons in the left panel, not exposed to the anti CGRP antibody, show the background grey level. Neurons in the other panels were counted as CGRP-positive if they had grey values above background. (D) Protocol of CGRP release experiments from sciatic nerve axons. Values from one nerve of a Scn11a +/+ mouse with 10 −7 M capsaicin are shown as an example. Areas in grey above the x-axis indicate the incubation time (in minutes) in the different test tubes. (E) CGRP release from sciatic nerve axons from naïve Scn11a +/+ mice without and after stimulation with different concentrations of capsaicin (n = 4–6 per concentration). (F) Scn11a +/L799P mice (3–7 months old) showed significantly less CGRP release from sciatic nerve axons in baseline (n = 14–21 nerves per group) and after stimulation with capsaicin (n = 8–11 nerves per group). Values are mean ± SEM. ***p < 0.001. BL baseline, CAPS capsaicin, CGRP calcitonin gene-related peptide, Δ difference.
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sandwich elisa kits item no. 10007610 81  (Bertin Pharma)
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Bertin Pharma sandwich elisa kits item no. 10007610 81
Salivary resistin, visfatin, and <t>ghrelin</t> in T2DM: Unstimulated whole saliva (UWS) was collected from 20 T2DM individuals and 20 healthy individuals. Each UWS sample was depleted of amylase and immunoglobulins. Precleaned UWS was assessed for (a) resistin, (b) visfatin, and (c) acylated and unacylated ghrelin using specific assay kits. ∗ p <0.05.
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Image Search Results


(A) Proportions of DRG neurons with CGRP-like immunoreactivity in lumbar sections from Scn11a +/+ and Scn11a +/L799P mice (n = 5–7 mice per group). (B) Sections of lumbar DRGs. The left section contains CGRP-positive neurons (labeled by stars), the right section was processed without anti-CGRP antibody for control. (C) Display of all DRG neurons analyzed for CGRP-like immunoreactivity. Each neuron is defined by its radius and its grey value. Neurons in the left panel, not exposed to the anti CGRP antibody, show the background grey level. Neurons in the other panels were counted as CGRP-positive if they had grey values above background. (D) Protocol of CGRP release experiments from sciatic nerve axons. Values from one nerve of a Scn11a +/+ mouse with 10 −7 M capsaicin are shown as an example. Areas in grey above the x-axis indicate the incubation time (in minutes) in the different test tubes. (E) CGRP release from sciatic nerve axons from naïve Scn11a +/+ mice without and after stimulation with different concentrations of capsaicin (n = 4–6 per concentration). (F) Scn11a +/L799P mice (3–7 months old) showed significantly less CGRP release from sciatic nerve axons in baseline (n = 14–21 nerves per group) and after stimulation with capsaicin (n = 8–11 nerves per group). Values are mean ± SEM. ***p < 0.001. BL baseline, CAPS capsaicin, CGRP calcitonin gene-related peptide, Δ difference.

Journal: PLoS ONE

Article Title: Gain-of-function mutation in SCN11A causes itch and affects neurogenic inflammation and muscle function in Scn11a +/L799P mice

doi: 10.1371/journal.pone.0237101

Figure Lengend Snippet: (A) Proportions of DRG neurons with CGRP-like immunoreactivity in lumbar sections from Scn11a +/+ and Scn11a +/L799P mice (n = 5–7 mice per group). (B) Sections of lumbar DRGs. The left section contains CGRP-positive neurons (labeled by stars), the right section was processed without anti-CGRP antibody for control. (C) Display of all DRG neurons analyzed for CGRP-like immunoreactivity. Each neuron is defined by its radius and its grey value. Neurons in the left panel, not exposed to the anti CGRP antibody, show the background grey level. Neurons in the other panels were counted as CGRP-positive if they had grey values above background. (D) Protocol of CGRP release experiments from sciatic nerve axons. Values from one nerve of a Scn11a +/+ mouse with 10 −7 M capsaicin are shown as an example. Areas in grey above the x-axis indicate the incubation time (in minutes) in the different test tubes. (E) CGRP release from sciatic nerve axons from naïve Scn11a +/+ mice without and after stimulation with different concentrations of capsaicin (n = 4–6 per concentration). (F) Scn11a +/L799P mice (3–7 months old) showed significantly less CGRP release from sciatic nerve axons in baseline (n = 14–21 nerves per group) and after stimulation with capsaicin (n = 8–11 nerves per group). Values are mean ± SEM. ***p < 0.001. BL baseline, CAPS capsaicin, CGRP calcitonin gene-related peptide, Δ difference.

Article Snippet: The samples were supplemented with 5-fold concentrated CGRP ELISA buffer (bertin pharma, Montigny-le-Bretonneux, France) and immediately stored at -20 °C.

Techniques: Labeling, Control, Incubation, Concentration Assay

Salivary resistin, visfatin, and ghrelin in T2DM: Unstimulated whole saliva (UWS) was collected from 20 T2DM individuals and 20 healthy individuals. Each UWS sample was depleted of amylase and immunoglobulins. Precleaned UWS was assessed for (a) resistin, (b) visfatin, and (c) acylated and unacylated ghrelin using specific assay kits. ∗ p <0.05.

Journal: Biochemistry Research International

Article Title: Assessment of Salivary Adipokines Resistin, Visfatin, and Ghrelin as Type 2 Diabetes Mellitus Biomarkers

doi: 10.1155/2018/7463796

Figure Lengend Snippet: Salivary resistin, visfatin, and ghrelin in T2DM: Unstimulated whole saliva (UWS) was collected from 20 T2DM individuals and 20 healthy individuals. Each UWS sample was depleted of amylase and immunoglobulins. Precleaned UWS was assessed for (a) resistin, (b) visfatin, and (c) acylated and unacylated ghrelin using specific assay kits. ∗ p <0.05.

Article Snippet: Volume equal to 1 µg of total protein in precleaned UWS was assessed for resistin, visfatin, and ghrelin per using specific sandwich ELISA kits (item no. 10007610 81, part no. 579020-96, and part no. 10006306-96, resp. ; Bertin Pharma/Cayman Chemical, Ann Arbor, MI, USA) following manufacturer's instructions.

Techniques: